force-plate actimeter (fpa)  (Bioanalytical Systems Inc)

Journal: Neurobiology of learning and memory

Article Title: Assessing Complex Movement Behaviors in Rodent Models of Neurological Disorders

doi: 10.1016/j.nlm.2018.02.025

Figure Lengend Snippet: The BASi Force-Plate Actimeter modified for simultaneous video capture. In this modification, three sides of the Actimeter have been modified with mirrors to facilitate an unimpeded view of the behavioral area by a CCD camera that captures video of the subject. Force transducers capture high resolution information about the center of force and its magnitude and movement (noted in Figure 2).

Article Snippet: 1.1.1 Force-plate Actimetry Detailed analysis of locomotor activity, exploratory behaviors, stereotypy, tremor and ataxia are facilitated by apparatus such as the BASi Force-Plate Actimeter (FPA) (Bioanalytical Systems, Inc., Mount Vernon, IN), which provides a very rich data stream that records and quantifies minute details of movement.

Techniques: Modification

Journal: Neurobiology of learning and memory

Article Title: Assessing Complex Movement Behaviors in Rodent Models of Neurological Disorders

doi: 10.1016/j.nlm.2018.02.025

Figure Lengend Snippet: Behavioral testing arena for assessment of photophobia and phonophobia. Apparatus is a modified Force-Plate Actimeter (BASi) fitted with a light/dark place preference enclosure. The “dark” side features opaque walls and sound attenuation; the “light” side has lamps and speakers that provide computer-controlled delivery of stimuli. The actimeter floor allows precise measurement of locomotor activity and movement patterns of the rat to avoid intense light or sound. In the migraine model, after infusion of the dural stimulus (while the subject is in the behavioral chamber) conditions in the behavioral arena were modified in five-minute periods with 1) no external light or sound, 2) illumination of one side of the chamber to 250 lux, 3) no external light or sound, and 4) 75 dB white noise delivered to one side of the chamber. A: Force transducers. B. Sound-attenuated, dark side of divided chamber. C. Opaque insulating material used to cover all sides of B. D. Speakers for delivery of audio stimuli. E. Lamp for illumination of one side of the arena.

Article Snippet: 1.1.1 Force-plate Actimetry Detailed analysis of locomotor activity, exploratory behaviors, stereotypy, tremor and ataxia are facilitated by apparatus such as the BASi Force-Plate Actimeter (FPA) (Bioanalytical Systems, Inc., Mount Vernon, IN), which provides a very rich data stream that records and quantifies minute details of movement.

Techniques: Modification, Activity Assay

Journal: Neurobiology of learning and memory

Article Title: Assessing Complex Movement Behaviors in Rodent Models of Neurological Disorders

doi: 10.1016/j.nlm.2018.02.025

Figure Lengend Snippet: Representative example of recordings of position/time data of a naïve female rat‥ Data were collected using a BASi force-plate actimeter. Analyses of total distance traveled was calculated based on the change in position of the center of force recordings over time. Note that the position traces reflect the initial exploratory behavior in the force-plate arena, which generally diminishes over time, with alternating periods of locomotion and rest.

Article Snippet: 1.1.1 Force-plate Actimetry Detailed analysis of locomotor activity, exploratory behaviors, stereotypy, tremor and ataxia are facilitated by apparatus such as the BASi Force-Plate Actimeter (FPA) (Bioanalytical Systems, Inc., Mount Vernon, IN), which provides a very rich data stream that records and quantifies minute details of movement.

Techniques:

Journal: Neurobiology of learning and memory

Article Title: Assessing Complex Movement Behaviors in Rodent Models of Neurological Disorders

doi: 10.1016/j.nlm.2018.02.025

Figure Lengend Snippet: Comparison of a WT mouse (A) and a homozygous transgenic laminin α5 mouse (B). The mice were 12 weeks old at the time of this assay. The hyperactivity of the transgenic mouse was expressed as almost continuous high-speed locomotion characterized by both broader and tighter circular loops that could occur anywhere on the actimeter floor. The wild type mouse was normoactive, and its movement trajectories reflected runs and pauses with more movements along walls than across the center of the chamber and relatively few rapid reversals of direction. Each square represents the floor of a 42 × 42 cm actimeter and contains the center of force movement trajectory for 7.5 seconds. Time advances from top to bottom and from left to right (i.e., the top of column 2 picks up where the bottom of column 1 left off). Total recording session time was 20 minutes. Figure 10 is an enlarged image of frame 99 for the homozygous transgenic mouse (B, shaded frame).

Article Snippet: 1.1.1 Force-plate Actimetry Detailed analysis of locomotor activity, exploratory behaviors, stereotypy, tremor and ataxia are facilitated by apparatus such as the BASi Force-Plate Actimeter (FPA) (Bioanalytical Systems, Inc., Mount Vernon, IN), which provides a very rich data stream that records and quantifies minute details of movement.

Techniques: Comparison, Transgenic Assay

Journal: Scientific Reports

Article Title: Changes in motor behavior, neuropathology, and gut microbiota of a Batten disease mouse model following administration of acidified drinking water

doi: 10.1038/s41598-019-51488-z

Figure Lengend Snippet: Effects of acidified drinking water on behavioral parameters of six-month-old Cln3 −/− and wild type mice measured in a force-plate actimeter. Cln3 −/− and wild type (WT) male mice either were kept on non-acidified water or received acidified water from postnatal day 21 (weaning). At six months of age, mice were tested in a force-plate actimeter, which measures several behavioral parameters in freely moving animals. The force-plate actimeter recorded data for 20.14 minutes, in fifty-nine 20.48-second frames, averaging 1,024 data points in each frame. a,b) In six-month-old Cln3 −/− mice on non-acidified water, the focused stereotypy score ( a ) and average power (force distribution) over band 1 (0–5 Hz) ( b ) were abnormally high and acidified water reduced them to a level that was not statistically different from that displayed by WT mice receiving non-acidified water. In the case of focused stereotypy score, the improvement reached a complete restoration to the wild type level between 7.2 and 13.3 minutes of force-plate actimeter recording when the difference between Cln3 −/− mice on acidified and non-acidified water became statistically significant. The focused stereotypy score quantifies vertical activity including head bobbing, grooming, rearing and scratching, and the average power was calculated by fast-Fourier transformation on force variation. c , d) In six-month-old Cln3 −/− mice, acidified water markedly increased the distance traveled ( c ) and the area covered ( d ). ( e , f ) Weights of the mice assessed in the behavioral tests at three ( e ) and six months ( f ) of age. Symbols and bars in graphs ( a , d ) represent mean ± SEM (n = 12–18). Columns and bars in graphs ( e,f ) represent mean ± SEM and the symbols show the individual data (n = 12–18). Statistical significance for the force-plate actimeter data was determined by repeated measures 2-way ANOVA with Dunnett’s post-test ( a-d ): * p < 0.05, Cln3 −/− on Non-acidified vs. WT on Non-acidified; # p < 0.05, Cln3 −/− on Non-acidified vs. Cln3 −/− on Acidified; & p < 0.05, Cln3 −/− on Acidified vs. WT on Acidified. Weights of the mice were analyzed by 1-way ANOVA with Sidaks’s post-test ( e , f ): *p < 0.05.

Article Snippet: The force-plate actimeter (BASi, West Lafayette, IN) measures mouse behavior with much finer spatial and temporal resolution than laser based devices.

Techniques: Activity Assay, Transformation Assay